Images presented in the figures were filtered by a 1-pixel Gaussian filter to reduce noise. To quantify and compare the line profiles from #links# 2P and 2P-STED images, we used the Lorentzian function: y=y0+2A��4(x?xc)2+��2,where y0 and A are constants, xc is the center, and �� is the width of the curve. Two-color images are shown merged with and without linear unmixing using a plugin for ImageJ


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